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Resuscitation Quick Actions • First 2 Minutes

High-Acuity

Rectal Temperature Mandate

Rectal temp >= 38.0°C (100.4°F); axillary and tympanic readings are unreliable; a reported home rectal fever counts as a true fever

0–28 Days Full Workup

CBC, Blood Culture, Catheter Urinalysis + Culture, Lumbar Puncture (CSF cell count, protein, glucose, culture, enterovirus/HSV PCR)

0–28 Days Empiric Meds

Ampicillin 50 mg/kg IV q8h (covers Listeria & Enterococcus) + Ceftazidime 50 mg/kg IV q8h (or Gentamicin 2.5 mg/kg IV q8h)

Acyclovir Indications

Add Acyclovir 20 mg/kg IV q8h if: maternal history of HSV, vesicles, seizures, CSF pleocytosis, hypothermia, or transaminitis

Avoid Ceftriaxone in Neonates

DO NOT use Ceftriaxone in infants < 28 days (displaces bilirubin from albumin causing kernicterus; precipitates with IV calcium)

29–60 Days Low-Risk Biomarkers

Procalcitonin <= 0.5 ng/mL, CRP <= 20 mg/L, ANC <= 4000/mcL, and negative catheter UA -> Safe to defer LP and observe

Bottom-Line Clinical Pearl

Any infant <= 28 days with a rectal temperature >= 38.0°C (100.4°F) requires a full sepsis evaluation (blood culture, catheterized urinalysis/culture, and lumbar puncture) and immediate admission on IV Ampicillin + Ceftazidime/Gentamicin +/- Acyclovir. In infants aged 29–60 days, modern PECARN and AAP guidelines allow risk-stratification using inflammatory biomarkers (procalcitonin <= 0.5 ng/mL, CRP <= 20 mg/L, absolute neutrophil count <= 4,000) and clean catheter urinalysis to safely defer lumbar puncture and avoid hospital admission in low-risk patients. Never treat an ear thermometer reading as accurate; rectal temperature is the mandatory gold standard.

1. Pathophysiology & Microbiology by Age Bracket

Young infants have immature humoral and cell-mediated immunity, impaired neutrophil chemotaxis, deficient complement levels, and permeable blood-brain barriers, placing them at profound risk for invasive bacterial infections (IBIs): bacteremia, urinary tract infection (UTI), and bacterial meningitis. Physical examination is notoriously unreliable in neonates, who can present with subtle lethargy, poor feeding, or tachypnea without focal signs.

2. Neonatal & Infant Fever Stratification Criteria

Infant Age TierPrimary PathogensClinical Workup AlgorithmEmpiric Antimicrobial Regimen
0 to 28 Days (Neonatal)Group B Streptococcus (GBS), Escherichia coli, Listeria monocytogenes, Klebsiella, Herpes Simplex Virus (HSV-1/2)Mandatory Full Sepsis Workup: Blood culture, catheterized UA/culture, Lumbar Puncture (CSF analysis & viral PCR). Inpatient admission mandatory.Ampicillin (50 mg/kg IV q8h) + Ceftazidime (50 mg/kg IV q8h) OR Gentamicin (2.5 mg/kg IV q8h). Add Acyclovir (20 mg/kg IV q8h) if HSV risk factors present.
29 to 60 DaysEscherichia coli, Group B Streptococcus, Streptococcus pneumoniae, Neisseria meningitidisRisk-Stratified Workup: Catheter UA, blood culture, and inflammatory biomarkers (Procalcitonin, CRP, ANC). Lumbar puncture indicated if biomarkers abnormal or ill-appearing.If abnormal biomarkers or ill: Ceftriaxone (50 mg/kg IV; note: safe once > 28 days) +/- Ampicillin. If all biomarkers low-risk: can defer LP and manage outpatient with 24h follow-up.
61 to 90 DaysEscherichia coli (UTI is #1), Streptococcus pneumoniae, EnterovirusTargeted Workup: Catheterized urinalysis and culture. Blood culture/CBC only if unvaccinated or clinically unwell.Antipyretics; oral antibiotics if UTI identified; outpatient discharge if well-appearing and vaccinated.

3. Critical Pitfalls & Antimicrobial Cautions

Critical Pitfall / Contraindication

CEFTRIAXONE CONTRAINDICATION IN NEONATES (< 28 DAYS): Ceftriaxone is strictly contraindicated in neonates under 28 days of age. It competitively binds albumin and displaces bilirubin, causing free hyperbilirubinemia and kernicterus (bilirubin encephalopathy). Furthermore, when co-infused with calcium-containing solutions (e.g., parenteral nutrition, Ringer's), it forms insoluble calcium-ceftriaxone crystalline precipitates in the lungs and kidneys. Use Ceftazidime or Gentamicin instead.

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